Hyperthermia using magnetite cationic liposomes for hamster osteosarcoma

Fumiko Matsuoka¹ , Masashige Shinkai¹ , Hiroyuki Honda¹ , Tadahiko Kubo² , Takashi Sugita² and Takeshi Kobayashi¹

¹Department of Biotechnology, School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan

²Department of Orthopaedic Surgery, Hiroshima University School of Medicine, 2-3, Kasumi 1 chome, Minami-ku, Hiroshima 734-8551, Japan

author email corresponding author email

BioMagnetic Research and Technology 2004, 2:3doi:10.1186/1477-044X-2-3


Published:	25 March 2004

Abstract

Background

We have developed magnetite cationic liposomes (MCLs) and applied them to local hyperthermia as a mediator. MCLs have a positive charge and generate heat under an alternating magnetic field (AMF) by hysteresis loss. In this study, the effect of hyperthermia using MCLs was examined in an in vivo study of hamster osteosarcoma.

Method

MCLs were injected into the osteosarcoma and then subjected to an AMF.

Results

The tumor was heated at over 42°C, but other normal tissues were not heated as much. Complete regression was observed in 100% of the treated group hamsters, whereas no regression was observed in the control group hamsters. At day 12, the average tumor volume of the treated hamsters was about 1/1000 of that of the control hamsters. In the treated hamsters, no regrowth of osteosarcomas was observed over a period of 3 months after the complete regression.

Conclusion

These results suggest that this treatment is effective for osteosarcoma.