|
Examples of proteinases and peptidases purified by magnetic techniques |
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| Purified enzyme |
Source |
Magnetic carrier |
Affinity ligand |
Further details |
Reference |
|
|
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| Aminopeptidase |
Arabidopsis |
Amine-terminated magnetic beads |
N-1-Naphthylphthalamic acid |
KCl gradient elution |
[54] |
| Angiotensin-converting enzyme |
Pig lung membranes |
Dynabeads |
Polyclonal antibodies |
[57] |
|
| Bromelain |
Commercial preparation |
Polyacrylic acid – iron oxide magnetic nanoparticles |
Elution with KCl solution |
[59] |
|
| Caspase (recombinant, histidine-tailed) |
Human cells |
Magnetic agarose |
Ni-NTA |
Elution with SDS-PAGE buffer |
[60] |
| Chymotrypsin |
Commercial preparation |
Magnetic chitosan beads |
Elution with N-acetyl-D-tryptophan |
[62] |
|
| NIa-protease (recombinant, histidine-tagged) |
Plum Pox Virus |
Magnetic core and nickel-silica composite matrix |
Ni2+ |
Elution with imidazole containing buffer |
[36] |
| Proteinases |
Commercial sources |
Magnetic cross-linked erythrocytes |
Elution with low pH buffer |
[46] |
|
| Proteinase, bacterial (Savinase) |
Bacillus clausii |
Silanized magnetite particles |
Bacitracin |
[84] |
|
| Trypsin |
Porcine pancreatin |
Silanized magnetite particles |
p-Aminobenzamidine |
Elution with low pH buffer |
[50] |
| Porcine pancreatin |
Magnetic polymer particles |
Soybean trypsin inhibitor |
Elution with low pH solution |
[86] |
|
| Commercial preparation |
Silanized ferrite powder |
Soybean trypsin inhibitor |
[87] |
||
| Commercial preparation |
Magnetic κ-carrageenan particles |
Soybean trypsin inhibitor |
Elution with low pH solution, MSFB |
[88][89] |
|
| Commercial preparation |
Magnetic polyacrylamide beads |
Soybean trypsin inhibitor |
Magnetically stabilized fluidized beds |
[90] |
|
| Commercial preparation |
Magnetic chitosan particles |
Aprotinin |
Elution with low pH solution |
[91] |
|
| Commercial preparation |
Magnetic cross-linked erythrocytes |
Elution with low pH buffer; separation from large volume sample |
[19] |
||
| Urokinase |
Crude urokinase preparation |
Magnetic dextran, agarose, polyvinyl alcohol, polyhydroxyethyl methacrylate microspheres |
p-Aminobenzamide, L-arginine methyl ester, guanidine hexanoic acid or guanidine acetic acid |
[93] |
|
Safarik and Safarikova BioMagnetic Research and Technology 2004 2:7 doi:10.1186/1477-044X-2-7 |
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