|
Purification of lysozyme by magnetic techniques |
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| Purified enzyme |
Source |
Magnetic carrier |
Affinity ligand |
Further details |
Reference |
|
|
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| Lysozyme |
Hen egg white |
Magnetic chitin |
Elution with 0.01 M HCl |
[71] |
|
| Hen egg white |
Magnetic acetylated chitosan |
Elution with 0.01 M HCl |
[9] |
||
| Commercial preparation |
Magnetic poly(2-hydroxyethyl methacrylate) |
Cibacron Blue F3GA |
Elution with 1 M KSCN |
[72] |
|
| Magnetic chitosan beads |
Magnetically stabilized fluidized bed |
[73] |
|||
| Ornithodoros moubata |
Magnetic chitin |
Elution with alkaline, high salt buffer |
[74] |
||
| Commercial preparation |
Magnetic cross-linked polyvinylalcohol |
Cibacron blue 3GA |
Elution with high salt buffer |
[52] |
|
| Magnetite – polyacrylic acid nanoparticles |
Ion-exchange separation |
[75] |
|||
| Magnetic cross-linked polyvinylalcohol beads |
Adsorption study |
[76] |
|||
| Commercial preparation |
Magnetic agarose beads |
Cibacron blue 3GA |
Magnetically stabilized fluidized bed |
[77] |
|
| Magnetic chitosan |
Cibacron blue 3GA |
Study of adsorption properties |
[78] |
||
| Commercial preparation |
Ferrofluid modified sawdust |
Elution with 0.5 M NaCl |
[79] |
||
| Commercial preparation |
Nano-sized magnetic particles |
Elution with NaH2PO4 and NaSCN |
[80] |
||
| Lysozyme (recombinant, histidine-tailed) |
T4 |
BioMag, amine terminated |
Iminodiacetic acid charged with Cu2+ |
Elution with low pH buffer |
[81] |
Safarik and Safarikova BioMagnetic Research and Technology 2004 2:7 doi:10.1186/1477-044X-2-7 |
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