|
Examples of polysaccharide and disaccharide hydrolases purified by magnetic techniques |
|||||
| Purified enzyme |
Source |
Magnetic carrier |
Affinity ligand |
Further details |
Reference |
|
|
|||||
| α-Amylases |
Porcine pancreas, Bacillus subtilis, wheat germ |
Magnetic alginate beads |
Elution with 1 M maltose |
[4] |
|
| Bacillus amyloliquefaciens, porcine pancreas |
Magnetic alginate microbeads |
Elution with 1 M maltose |
[10] |
||
| β-Amylase |
Sweet potato |
Magnetic alginate beads |
Elution with 1 M maltose |
[55] |
|
| β-Galactosidase |
Escherichia coli homogenate |
Silanized magnetite |
p-Aminophenyl-β-D-thiogalactopyranoside |
Elution with borate buffer, pH 10 |
[58] |
| β-Galactosidase (fusion protein comprising the DNA-binding lac repressor) |
Bacterial lysate |
Magnetic beads |
DNA containing Escherichia coli lac operator |
Elution with lactose analogue |
[64] |
| Glucoamylase |
Aspergillus niger |
Magnetic alginate beads |
Elution with 1 M maltose |
[55] |
|
| Pectinase |
Commercial preparation |
Magnetic alginate beads |
[82] |
||
| Pullulanase |
Bacillus acidopullulyticus |
Magnetic alginate beads |
Elution with 1 M maltose |
[55] |
|
Safarik and Safarikova BioMagnetic Research and Technology 2004 2:7 doi:10.1186/1477-044X-2-7 |
|||||