Table 4 |
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|
Examples of other enzymes purified by magnetic techniques |
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| Purified enzyme |
Source |
Magnetic carrier |
Affinity ligand |
Further details |
Reference |
|
|
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| Alcohol dehydrogenase |
Yeast homogenate |
Magnetic cross-linked polyvinylalcohol |
Cibacron blue 3GA |
Elution with high salt buffer |
[52] |
| Saccharomyces cerevisiae extract |
PEG with bound Cibacron blue |
Magnetic two-phase system |
[53] |
||
| Aldolase (recombinant, histidine tagged) |
Pea |
Magnetic core and nickel-silica composite matrix |
Ni2+ |
Elution with imidazole containing buffer |
[36] |
| AngioI-TEM-β-lactamase |
Escherichia coli cells extracts |
Magnetic agarose beads |
Iminodiacetic acid charged with Zn2+ |
Elution with low pH buffer |
[56] |
| Asparaginase |
Escherichia coli homogenate |
Magnetic polyacrylamide gel particles |
D-Asparagine |
Elution with D-asparagine solution |
[58] |
| Carbonic anhydrase |
Model mixture |
Magnetic agarose beads |
Sulfanilimide |
Elution with high salt buffer |
[14] |
| Catalase |
Bovine liver, commercial preparation |
Magnetic poly(EGDMA-MAH) beads |
Fe3+ |
Elution with NaSCN solution |
[61] |
| Cytochrome c |
Horse, Candida krusei |
Amine terminated iron oxide particles |
Iminodiacetic acid charged with Cu2+ |
Binding studies |
[63] |
| Commercial preparation |
Au@magnetic particles |
MALDI MS analysis |
[31] |
||
| Horse heart |
Magnetic agarose beads |
Iminodiacetic acid charged with Cu2+ |
Elution with EDTA containing buffer |
[56] |
|
| Bovine heart |
Magnetic ion-exchange particles |
Protein binding studies |
[12] |
||
| Glucose-6-phosphate dehydrogenase |
Ferrofluid modified Sepharose 4B |
ADP |
[65] |
||
| Saccharomyces cerevisiae extract |
PEG with bound Cibacron blue |
Magnetic two-phase system |
[53] |
||
| Hexokinase |
Escherichia coli homogenate |
PEG with bound Cibacron blue |
Magnetic two-phase system |
[53] |
|
| Lactate dehydrogenase |
Beef heart |
Ferrofluid modified Sepharose 4B |
AMP |
Elution with 1 mM NADH |
[13] |
| Porcine muscle |
Magnetic agarose beads |
Reactive Red 120 |
Column elution with NaCl gradient |
[66] |
|
| Lactoperoxidase |
Sweet whey |
Magnetic cation exchanger |
HGMS |
[67,68] |
|
| Luciferase (histidine-tagged) |
Escherichia coli homogenate |
MagneHis™ system |
Ni2+ |
[69,70] |
|
| Phosphatase, alkaline |
Human placenta |
Dynabeads M-450 |
Specific antibody |
Activity of bound enzyme measured |
[83] |
| Phosphatase, alkaline (fusion protein comprising the DNA-binding lac repressor) |
Bacterial lysate |
Magnetic beads |
DNA containing Escherichia coli lac operator |
Elution with lactose analogue |
[64] |
| Phosphofructokinase |
Saccharomyces cerevisiae extract |
PEG with bound Cibacron blue |
Magnetic two-phase system |
[53] |
|
| 6-Phosphogluconate dehydrogenase |
Ferrofluid modified Sepharose 4B |
ADP |
Elution with 1 mM NADP |
[13] |
|
| Thioredoxin (recombinant, histidine-tagged) |
Escherichia coli |
Magnetic agarose |
Ni-NTA |
Elution with imidazole containing buffer |
[20] |
| tRNA methionyl synthetase (recombinant, histidine-tagged) |
Escherichia coli |
MagneHis™ system |
Ni2+ |
Rapid detection and quantitation of isolated protein |
[85] |
| Uricase (recombinant, histidine-tailed) |
Bacillus |
Ion-chelating magnetic agarose beads |
Ni2+ |
Elution by cleavage with proteinase K |
[92] |
|
|
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|
Safarik and Safarikova BioMagnetic Research and Technology 2004 2:7 doi:10.1186/1477-044X-2-7 |
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