|
Examples of antibodies purified by magnetic techniques |
|||||
| Purified antibody |
Source |
Magnetic carrier |
Affinity ligand |
Further details |
Reference |
|
|
|||||
| Anti-BODIPY-fluorescein antibodies |
Magnetoliposomes |
BODIPY-fluorescein |
[94] |
||
| Anti-DNA antibody |
Systemic lupus erythematosus patient plasma |
Magnetic poly(2-hydroxyethyl-methacrylate) beads |
DNA |
Desorption with 1 M NaSCN solution |
[95] |
| Anti-human chorionic gonadotropin antibody |
Murine ascites supernatants |
Magnetic cellulose beads |
Human chorionic gonadotropin |
[96] |
|
| Antibody (from rat) |
Sample from affinity chromatography |
Dynabeads M-280 |
Sheep anti-rabbit IgG |
Antibody concentration |
[3] |
| Antibody |
Rabbit serum |
Dynabeads M-280 |
Sheep anti-rabbit IgG |
Elution with 0.5 M acetic acid |
[97] |
| Monoclonal antibodies |
Mouse hybridoma culture broth |
Magnetite particles |
Protein A |
[98] |
|
| Anti-bovine serum albumin antibodies |
Thermosensitive magnetic microspheres |
Bovine serum albumin |
Immobilization by the carbodiimide method |
[99] |
|
| Immunoglobulin G, human |
Commercial preparation |
Magnetic poly(ethylene glycol dimethacrylate-N-methacryloly-L-histidine-methylester) beads |
Elution with 1 M NaCl |
[100] |
|
| Immunoglobulin G |
Blood serum |
Carboxyl-terminated magnetic particles |
MproteinAG |
[101] |
|
| IgE antibodies |
Allergic patients sera |
Magnetoliposomes |
Antigenic proteins |
[16] |
|
| Murine anti-fibroblast growth factor receptor 1 IgM |
Ascites |
Polystyrene magnetic beads |
Rat anti-mouse IgM monoclonal antibody |
[102] |
|
Safarik and Safarikova BioMagnetic Research and Technology 2004 2:7 doi:10.1186/1477-044X-2-7 |
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