Table 6

Examples of DNA/RNA/oligonucleotide/aptamer binding proteins purified by magnetic techniques
Purified protein	Source	Magnetic carrier	Affinity ligand	Further details	Reference

CUG binding proteins	Human myoblasts or fibroblasts	Dynabeads M-280 streptavidin	Biotinylated(CUG)₁₀	Elution with 1 M NaCl	[103]
Transcription factor τ	Saccharomyces cerevisiae	Dynabeads M-280 streptavidin	Biotinylated tRNA^Glugene fragment	Elution with high salt buffer	[104,105]
DNA-binding proteins	Crude tissue extract	Magnetic phospho cellulose particles			[106]
DNA-binding proteins	Escherichia coli	Magnetic phospho cellulose particles			[107]
DNA-binding proteins	HeLa nuclear extracts	Dynabeads M-280 streptavidin	Biotin-labelled DNA fragment	Elution with 2 M NaCl	[108]
Vaccinia virus early transcription factor	Vaccinia virions	Dynabeads M-280 streptavidin	Biotinylated double-stranded DNA	Elution with high salt buffer	[109]
Ecdysteroid receptor	Drosophila melanogaster nuclear extract	Dynabeads M-280 streptavidin	Biotinylated double-stranded oligonucleotide	Elution with 0.4 M KCl	[110]
NanR protein (recombinant)	Escherichia coli	μMACS streptavidin MicroBeads	Biotin-labelled DNA fragment	Elution with 1 M NaCl	[111]
p27	Rabbit hepatocytes	Dynabeads M-280 streptavidin	Guanine-rich single-stranded DNA	Elution with NaCl solution	[112]
Pigpen protein	Endothelial cells	Magnetic streptavidin beads	Biotinylated aptamer	Elution with 1 M NaCl	[113]
RNA binding proteins	Saccharomyces cerevisiae	μMACS streptavidin MicroBeads	Biotin-labelled RNA probe	Elution with 1 M NaCl	[114]
Single-stranded telomere binding protein (sTBP)	Nuclei from vertebrate tissues	Dynabeads M-280 streptavidin	Biotinylated single stranded TTAGGG_nrepeats	Elution with high salt buffer	[115]
Transcription proteins	Human myeloid cells	Dynabeads M-280 streptavidin	Biotinylated serum inducible element (hSIE)	Elution with high salt buffer	[116]
Transcription factor γRF-1	Human monocytes and epidermal cells	Dynabeads M-280 streptavidin	Biotinylated DNA containing γRF-1 sequences	Elution with 0.6 M KCl	[117]
Protein factor MS2	Murine skeletal myotubes	Dynabeads	Double-stranded DNA	Elution with 100 mM sodium acetate, pH 4.2	[118]
Guide RNA binding protein	Trypanosoma brucei mitochondria	Dynabeads M-450 goat anti-mouse IgG	Monoclonal antibody	Elution with low pH buffer cont. SDS	[119]
RNA binding proteins	Pollen grains	Streptavidin MagneSphere particles	Biotinylated oligonucleotides	Elution with SDS buffer	[120]
DNA binding protein	Schistosoma mansoni	Dynabeads M-280 streptavidin	Biotinylated DNA	Elution with sodium acetate buffer	[121]
ssDNA binding proteins	Transfected mouse fibroblasts	Dynabeads anti-rabbit IgG	Rabbit antibody	Indirect method	[122]
Tenascin-C	Glioblastoma cells	Dynabeads streptavidin	Biotinylated aptamer	Elution with high salt buffer	[123]
Thermostable brain factor (ThBF)	Rat brain	Streptavidin magnetic particles	Biotinylated oligonucleotides	Elution with 0.7 M KCl	[124]
TTF1 protein	Escherichia coli lysate	Dynabeads M-280 streptavidin	Biotinylated aptamer	Elution with DNase	[125]

Safarik and Safarikova BioMagnetic Research and Technology 2004 2:7 doi:10.1186/1477-044X-2-7