|
Purification of albumin and haemoglobin by magnetic techniques |
|||||
| Purified protein |
Source |
Magnetic carrier |
Affinity ligand |
Further details |
Reference |
|
|
|||||
| Albumin, bovine serum |
Commercial preparation |
Magnetic agar beads |
Cibacron blue3GA |
Adsorption experiments |
[126] |
| Commercial preparation |
Magnetic cross-linked polyvinylalcohol |
Cibacron blue3GA |
Adsorption experiments |
[76,127] |
|
| Magnetic chitosan microspheres |
Cibacron blue3GA |
[78] |
|||
| Commercial preparation |
Magnetic poly(glycidyl methacrylate-triallyl isocyanurate-divinylbenzene) particles |
Anion exchange separation |
[128] |
||
| Commercial preparation |
Magnetic poly(ethylene glycol dimethacrylate-co-N-methacryloyl-(L)-histidine methyl ester) microbeads |
Cu2+ |
Elution with 1.0 M NaSCN |
[129] |
|
| Albumin, human serum |
Commercial preparation |
Magnetic poly(2-hydroxyethylmethacrylate) beads |
Iminodiacetic acid charged with Cu2+ |
Elution with 1.0 M NaSCN |
[130] |
| Human plasma |
Magnetic poly(2-hydroxyethyl methacrylate) beads |
Cibacron blue F3GA |
Elution with 0.5 M NaSCN |
[131] |
|
| Commercial preparation |
Magnetic particles covered with thermosensitive polymer |
- |
Desorption by decreasing temperature |
[132,133] |
|
| Albumin, human serum (recombinant, FLAG tagged) |
Yeast cells |
Magnetic glass beads |
Anti-FLAG antibody |
Elution with EDTA containing buffer |
[1] |
| Glycated haemoglobin |
Human blood |
Magnetic poly(vinyl alcohol) beads |
m-Aminophenyl-boronic acid |
Elution with sorbitol |
[138] |
| Haemoglobin |
Bovine, commercial preparation |
Amine terminated iron oxide particles |
Iminodiacetic acid charged with Cu2+ |
Elution with imidazole containing buffer |
[63] |
| Haemoglobin A1c |
Human blood |
Magnetic particles isolated from Magnetospirillum magneticum AMB-1 |
m-Aminophenyl-boronic acid |
used for affinity immunoassay |
[150] |
Safarik and Safarikova BioMagnetic Research and Technology 2004 2:7 doi:10.1186/1477-044X-2-7 |
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