Table 9 |
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|
Examples of peptides purified by magnetic techniques |
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| Purified peptide |
Source |
Magnetic carrier |
Affinity ligand |
Further details |
Reference |
|
|
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| Biotinylated peptides |
Model mixtures |
Dynabeads M-280 streptavidin |
Streptavidin |
Used in MALDI-TOF mass analysis |
[159] |
| (His)6-Ala-Tyr-Gly |
Synthetic peptide |
Dynabeads M-280 tosylactivated |
Aminocaproic nitrilotriacetic acid charged with Ni2+ |
Elution with imidazole solution |
[160] |
| Synthetic pentapeptides against fructose-1,6-biphosphate aldolase |
Synthetic mixture |
Streptavidin-coated magnetic beads |
Biotin labelled fructose-1,6-biphosphate aldolase of T. brucei |
Pentapeptides were anchored on polystyrene beads |
[161] |
| Tryptic digest products of cytochrome c |
Trypsin digested cytochrome c |
Au@magnetic particles |
- |
Ion-exchange separation followed by MALDI MS analysis |
[31] |
| Glutathione |
Gold and iron oxide nanocomposites |
[162] |
|||
| Nisin Z |
Lactobacillus lactis |
EDC activated magnetic beads |
Anti-nisin antibody |
Elution with 6 M urea |
[163] |
|
|
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|
Safarik and Safarikova BioMagnetic Research and Technology 2004 2:7 doi:10.1186/1477-044X-2-7 |
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